Water Conditioning & Purification Magazine

Biological Water: A Flexible Designer Fluid?

By Martin Gruebele, PhD

Water is just water: a well-known fluid that freezes at 32°F (0°C) and boils at 212°F (100°C) , as long as we keep it under one atmosphere of pressure and add no osmolytes to depress its freezing point or elevate its boiling point.

That may be true in the bulk, but not when water is forced into nanometer-size pores or surrounded by large biomolecules, such as proteins. In those cases, an array of modern spectroscopic measurement methods tells us that water properties can be tailored extensively. As exotic as such conditions may seem, they occur everywhere around us, from water purifiers based on ceramic filters to water contaminated by biological residues.

Nano-confined water molecules can be made to rotate or diffuse more rapidly or they can be frozen into nano-sized ice sheets. Near biocontaminants, water absorbs low-frequency radiation more strongly, where normally it would have been transparent. Even water density can be lowered well below a gram/cm3. The design possibilities are endless.

Modern spectroscopic detection techniques can be used to monitor the unusual properties of water that is confined in pores, or between biomolecules or stretched thin. In the examples discussed here, water has properties remarkably altered from the bulk fluid we are all familiar with.

Water pairing

Water pairing up with the protein ubiquitin is a good example. Ubiquitin is aptly named because this protein occurs in large quantities in all human cells, where it helps recycle other ‘used’ proteins. It serves as a good prototype of how large biomolecules interact with water.

Figure 1 highlights a large zone of water around ubiquitin that no longer behaves like the bulk. This is not just idle scientific play: once taken up by a living organism, water is never more than a few nanometers from a large biomolecule such as a protein (about 1:250,000,000th of an inch, to put it in perspective).

Likewise, groundwater stored in clays is never far from the surface of a pore and we use clays and zeolites to purify water. Almost all the water in such pores or near large biomolecules is different from the bulk. This can create problems; if water is less mobile in such pores, the transport of contaminants could be hindered and they could be trapped, only to be released later when temperature or pressure conditions change.

Magnetic resonance

To look at the effects of water immobilization in pores, Jiri Jonas and co-workers investigated the motion of water in kaolinites, silica glasses and other nanoporous materials in the 1990s.1 They used nuclear magnetic resonance spectroscopy, similar to MRI imaging of the human brain at hospitals today, to look at how water molecules move around in pores.

The idea is that the two hydrogen atoms in water produce a small magnetic field. A large magnetic field pulse applied by the experimenter can align these magnetic fields temporarily, but when the pulse is turned off, they relax back to their original value.

The time it takes to relax tells us how quickly water molecules tumble and reorient. Nuclear magnetic resonance uses radio waves (about 500 Megahertz) to detect that relaxation, not far from the frequency of radios or cordless phones.

Jonas and his colleagues found that the wetting layer of water near the wall of the nanopore forms regular sheets with most of the oxygen atoms pointing in the same direction and that these sheets hinder the diffusion of other molecules dissolved in water. Water has a volume of about 12 Å3 and a moderate-sized cavity in a zeolite (such as used in water purification or laundry detergents) is about 120 Å3.

When ten water molecules are packed into such a cavity, all but one would be in the wetting layer on average. Ninety percent of the water molecules in a filter do not transport contaminants in the way expected from the bulk. They may either slow down the diffusion of contaminants or in some cases, by excluding them from the surface layer, they could speed up diffusion.

Thus, tailoring how water interacts with the filter material could have a significant effect on how impurities are retained or flushed through. Design properties at the molecular scale have a direct effect on the large-scale behavior.

Terahertz spectroscopy

Biomolecules are an important contaminant of water, from bacterial metabolic products to human food wastes. One could use nuclear magnetic resonance to see how biomolecules affect water mobility, but the technique is only sensitive to one or two layers of water on the surface of a biomolecule, when the effect could be far ranging.

Now we fast-forward from 1990 to 2008, when a new technique for studying water in the presence of biomolecules has become available: Terahertz or far-infrared spectroscopy. This form of spectroscopy uses electromagnetic waves with a frequency of about 10,000,000 Megahertz (or 10 Terahertz) a considerably higher frequency than radio- or microwaves, but still much lower than infrared or visible light.

Far-infrared light has been used in research laboratories for many decades, but only recently have laser-driven Terahertz systems become powerful enough to probe water. The reason is that water absorbs Terahertz light much more strongly than radiowaves or microwaves do.2

A typical microwave oven penetrates a few inches into a layer of water, a three-Terahertz beam only about a thousandth of an inch. That makes it harder to get the Terahertz beam into the water to find out how water molecules are moving around.

Interestingly enough, ice is almost completely transparent to Terahertz light. The reason is that the vibrational motions of liquid water are locked down in ice because a full complement of four rigid hydrogen bonds forms between water molecules.

The radiation cannot excite the water molecules any longer, so the ice is transparent. But biomolecules have quite the opposite effect. Pure protein absorbs Terahertz light even less than water, but when mixing the two, one gets more absorption of Terahertz light than from either water or protein alone.3

What is the explanation? The protein actually makes water within a one to two nanometer layer near its surface move about more flexibly than it did before, at least at a frequency of three Terahertz. Thus, water near a protein is more absorbing and less transparent.

Protein folding

The nanometer distance range is very typical of the distance between biomolecules in a living cell, so water molecules cannot escape the influence of proteins, carbohydrates and nucleic acids. Even the blood stream is filled with plasma proteins and carbohydrates. There is very little bulk water in living things.

Just like zeolite or clay nanopores, biomolecules modify the way water moves on a picosecond time scale. Picoseconds may seem so fast that one would not care, but a cascade of motion, referred to as ‘slaving of slower motions to fast motions’ by Hans Frauenfelder, means that those picosecond events ultimately affect much slower time scales, such as when water and biomolecules mix or their separation is attempted.

We now have instruments that can directly follow the very fast dynamics of water molecules as they dance together with biomolecules. Kinetic Terahertz Absorption Spectroscopy (KITA) monitors Terahertz pulses going through a water-protein solution with high-time resolution, following the rearrangements of the water layers surrounding a protein from picoseconds to seconds.

Water motion turns out to be very important for the process of protein folding. Protein folding, during which a chain of amino acids folds into a well-defined structure (as in Figure 1), occurs in all organisms after a protein has been made by the ribosome from amino acids.

The total energy by which a folded protein is stabilized compared to the unfolded protein is tiny; it corresponds to just two to four water hydrogen bonds. The motion of hydration water molecules guides proteins during folding and, likewise, the protein changes the dynamics of water molecules in a mutual dance.

Figure 2 shows a schematic of the KITA instrument. It took the water molecules only a few milliseconds to adapt to the changing shape of the protein.4 On the other hand, fluorescence spectroscopy reveals that the complete folding process takes over a second. Thus, while folding to the final structure of a protein may be relatively slow, the early stages, guided by water rearrangement, are quite fast. The water shell around proteins helps kick-start the folding process.

Stretching water

Water can visibly alter the stability of biological molecules it comes into contact with and no process highlights this better than artificially stretching water that contains protein molecules. To this end, physics graduate student Edgar Larios (in my research group) adapted a clever idea invented by Berthelot in 1850.5

Larios put water and protein into a tube, pumped on the liquid with a vacuum pump to make sure no air remained and then sealed the tube, leaving a small vapor bubble at the top. He then heated the tube, making the bubble disappear by the expansion of the water.

But when he cooled down the tube, the bubble did not reappear. Instead, the water stuck to the walls of the glass tube because of surface tension and was stretched to a density of about 0.95 grams/cm3, as opposed to the usual one gram/cm3. The protein inside this stretched water became less stable than in normal water.

We know this by doing nuclear magnetic resonance spectroscopy and observing the relative size of two peaks in the spectrum: one peak corresponds to the unfolded protein, another to the folded protein. The relative strength of these peaks switched when the water was stretched (Figure 3).

To summarize, pores and biomolecules have a profound effect on the microscopic motions of water and vice-versa. These motions can be amplified to the macroscopic scale, so water is not a passive solvent.

This knowledge could be used to design better porous materials at the molecular level, affecting how biological contaminants are transported and filtered through pores. Water is a truly flexible fluid, so the design possibilities are endless.

References

  1. J. P. Korb, L. Malier, F. Cros, et al., Physical Review Letters 77, 2312 (1996).
  2. S. Dexheimer, Terahertz spectroscopy: principles and applications (Taylor & Francis, London, 2007).
  3. S. Ebbinghaus, S. J. Kim, M. Heyden, et al., Journal of American Chemical Society 130, 2374 (2008).
  4. S. J. Kim, B. Born, M. Havenith, et al., Angew. Chemie International Ed. 47, 6486 (2008).

M. Berthelot, Annales des Chimie et des Physique 30, 232 (1850).

About the author

Dr. Martin Gruebele is with the Departments of Chemistry, Physics and Biophysics and Computational Biology at the University of Illinois, Urbana-Champaign. Earning a Ph.D. from the University of California, Berkeley in 1988, Dr. Gruebele focuses on experimental and theoretical understanding of the dynamics of complex molecular systems, including those of large molecular size and their interaction with their environment.

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